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细胞表面修饰是工程化细胞的关键策略之一，对研究细胞功能及拓展其应用具有重要意义。文章通过迈克尔加成反应制备了一种含有Kim-1靶向肽(KBP)的DMPE-PEG-KBP(DMPK)磷脂分子，并利用非共价疏水相互作用将DMPK修饰到间充质干细胞(MSCs)表面。通过共聚焦显微镜，系统考察了DMPK浓度、修饰时间、修饰温度以及修饰环境对表面修饰效果的影响，同时评估了修饰对MSCs行为的影响。实验结果表明，DMPK能在MSCs表面实现均匀修饰，且不影响MSCs的增殖能力及表型特征。本研究为干细胞工程化修饰提供了一种可行方法，对推动干细胞的相关应用具有重要价值。
Abstract：Cell surface modification is one of the main methods of engineering cells, which is of great significance for the study of cell function and application. In this paper, a DMPE-PEG-KBP(DMPK) phospholipid molecule containing Kim-1 targeting peptide(KBP) was prepared by Michael addition reaction, and DMPK was modified on the surface of mesenchymal stem cells(MSCs) by non-covalent hydrophobic interaction. The effects of DMPK concentration, modification time, modification temperature and modification environment on the modification were investigated by confocal microscopy. The effects of modification on the behavior of MSCs were also studied. The results showed that DMPK could modify the surface of MSC uniformly, and had no effect on the proliferation and phenotype of MSCs. This study provides an effective method for the engineering modification of stem cells and has important value for the development of stem cell applications.
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基本信息:
DOI：10.16026/j.cnki.iea.2026010032
中图分类号:Q2-33
引用信息:
[1]胡雅琦,李茹祥,牛晓媛,等.功能性磷脂分子修饰细胞膜及对细胞行为的影响[J].离子交换与吸附,2026,42(01):32-38.DOI:10.16026/j.cnki.iea.2026010032.
基金信息:
国家重点研发计划(项目号2020YFA0710801)
2026-02-20
2026-02-20