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本文利用苯硼酸基团与细菌之间的特异性黏附，将苯硼酸衍生物苯硼酸-[3,4-二氢嘧啶-2(1H)]-酮(PBA-DHPM)与乳糖酰胺基乙基甲基丙烯(LAMA)单体共聚，制备了细菌响应性药物释放纳米平台，采用核磁共振氢谱1H-NMR对p(PBA-DHPM-r-LAMA)共聚物的结构进行表征。通过自组装过程制备了细菌响应性两亲性纳米载体后，本实验采取动态光散射及透射电子显微镜观察纳米颗粒的粒径及外观形貌，并通过细胞毒性及体外释放实验，验证该苯硼酸基纳米载体的生物相容性及细菌响应性释放性能。实验证明，该纳米载体的载药能力强，粒径均一且具有良好的生物相容性及细菌响应性药物释放性能。
Abstract：In this paper, a responsive drug-release nanoplatform was prepared using phenylboronic acid-[3,4-dihydropyrimidin-2(1H)]-one(PBA-DHPM), which has bacterialspecific adhesion properties, copolymerized with lactosaminyl ethyl methacrylate(LAMA)monomer. The structures of p(PBA-DHPM-r-LAMA) copolymers were characterized by1H-NMR. After the bacterially responsive amphiphilic nanocarriers were prepared by the selfassembly process, dynamic light scattering and transmission electron microscopy were utilized to observe the particle size and appearance morphology of the nanoparticles, and the bacterially responsive release performance of the phenylboronic acid-based nanocarriers was verified by in vitro release experiments. It was demonstrated that the nanotherapeutics have high drug loading capacity, uniform particle size, and superior biocompatibility and bacterial responsive drug release performance.
[1]Li Zhiyu,Luo Bin,Yu Lingzhu,Lan Fang,Wu Yao.Intermolecular B-N coordination and multi-interaction synergism induced selective glycoprotein adsorption by phenylboronic acid-functionalized magnetic composites under acidic and neutral conditions[J].Journal o Materials Chemistry B,2021,9(2):453-463.
[2]Obuobi Sybil,Voo Zhi Xiang,Low Mei W,Czarny Bertrand,Selvarajan Vanitha,Ibrahim Nor L,Yang Yi Yan,Ee Pui Lai Rachel.Phenylboronic acid functionalized polycarbonate hydrogels for controlled release of polymyxin B in pseudomonas aeruginosa infected burn wounds[J].Advanced Healthcare Materials,2018,7(13):Art no e1701388.
[3]Gaber Mohamed,Hany Mark,Mokhtar Sarah,Helmy Maged W,Elkodairy Kadria AElzoghby Ahmed O.Boronic-targeted albumin-shell oily-core nanocapsules for synergistic aromatase inhibitor/herbal breast cancer therapy[J].Materials Science&Engineering C-Materials for Biological Applications,2019,105:Art no 110099.
[4]Qian Xiaoping,Ge Lei,Yuan Kangjun,Li Cheng,Zhen Xu,Cai Weibo,Cheng Rongshi Jiang Xiqun.Targeting and microenvironment-improving of phenylboronic acid-decorated soy protein nanoparticles with different sizes to tumor[J].Theranostics,2019,9(24):7417-7430.
[5]Elshaarani Tarig,Yu Haojie,Wang Li,Zain-ul-Abdin,Ullah Raja Summe,Haroon Muhammad,Khan Rizwan Ullah Fahad,Shah Khan Amin,Nazir Ahsan,Usman Muhammad,Naveed Kaleem-ur-Rahman.Synthesis of hydrogel-bearing phenylboronic acid moieties and their applications in glucose sensing and insulin delivery[J].Journal o Materials Chemistry B,2018,6(23):3831-3854.
[6]Dautta Manik,Alshetaiwi Muhannad,Escobar Jens,Tseng Peter.Passive and wireless implantable glucose sensing with phenylboronic acid hydrogel-interlayer RF resonators[J].Biosens Bioelectron,2020,151:Art no 112004.
[7]Huang Qiao,Wang Li,Yu Haojie,Ur-Rahman Kaleem.Advances in phenylboronic acidbased closed-loop smart drug delivery system for diabetic therapy[J].Journal of Controlled Release,2019,305:50-64.
[8]Tan Guoxin,Li Jinyu,Song Yiming,Yu Yibin,Liu Dandan,Pan Weisan.Henylboronic acidtethered chondroitin sulfate-based mucoadhesive nanostructured lipid carriers for the treatment of dry eye syndrome[J].Acta Biomaterialia,2019,99:350-362.
[9]Beikmohammadi Hanie,Viesy Soghra,Kaviani Rasoul,Pouladi Iman.Detection of efflux pump genes conferring multidrug resistance in clinical isolates of Acinetobacter Baumanni in Tehran province[J].Reviews in Medical Microbiology,2022,33(1):31-36.
[10]Liu Jiayong,Li Hongxia,Li Haizhou,Fang Shanfang,Shi Jinguo,Chen Yongzhi,Zhong Rongcui,Liu Shouping,Lin Shuimu.Rational design of dipicolylamine-containing carbazole amphiphiles combined with Zn2+as potent broad-spectrum antibacterial agents with a membrane-disruptive mechanism[J].Journal of Medicinal Chemistry,2021,64(14):10429-10444.
[11]Hussain Sazid,Joo Jinmyoung,Kang Jinyoung,Kim Byungji,Braun Gary B,She Zhigang Kim Dokyoung,Mann Aman P,Molder Tarmo,Teesalu Tambet,Carnazza Santina Guglielmino Salvatore,Sailor Michael J,Ruoslahti Erkki.Antibiotic-loaded nanoparticles targeted to the site of infection enhance antibacterial efficacy[J].Nature Biomedica Engineering,2018,2(2):95-103.
[12]Yang Shengbing,Han Xiuguo,Yang Ying,Qiao Han,Yu Zhifeng,Liu Yang,Wang Jing Tang Tingting.Bacteria-targeting nanoparticles with microenvironment-responsive antibiotic release to eliminate intracellular Staphylococcus aureus and associated infection[J].ACS Applied Materials&Interfaces,2018,10(17):14299-14311.
[13]Zhao Yu,Yu Cong,Yu Yunjian,Wei Xiaosong,Duan Xiaozhuang,Dai Xijuan,Zhang Xinge.Bioinspired heteromultivalent ligand-decorated nanotherapeutic for enhanced photothermal and photodynamic therapy of antibiotic-resistant bacterial Pneumonia[J].ACS Applied Materials&Interfaces,2019,11(43):39648-39661.
[14]Eissa Ahmed M,Abdulkarim Ali,Sharples Gary J,Cameron Neil R.Glycosylated nanoparticles as efficient antimicrobial delivery agents[J].Biomacromolecules,2016,17(8):2672-2679.
[15]Dai Xiaomei,Zhao Yu,Yu Yunjian,Chen Xuelei,Wei Xiaosong,Zhang Xinge,LChaoxing.All-in-one NIR-activated nanoplatforms for enhanced bacterial biofilm eradication[J].Nanoscale,2018,10(39):18520-18530.
[16]Zhang Canyang,Gao Jin,Wang Zhenjia.Bioresponsive nanoparticles targeted to infectious microenvironments for sepsis management[J].Advanced Materials,2018,30(43):Art no e1803618.
基本信息:
DOI：10.16026/j.cnki.iea.2022020097
中图分类号:TQ460.4;TB383.1
引用信息:
[1]张艳龙,李刚,张新歌,等.细菌响应性纳米载体的构建及性能研究[J].离子交换与吸附,2022,38(02):97-105.DOI:10.16026/j.cnki.iea.2022020097.
基金信息:
国家自然科学基金(编号:21975133,21774062)
2022-04-20
2022-04-20