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组织再生中缺氧现象的存在是影响再生性能的重要原因。氧气在组织再生中具有重要作用,支架材料中心部位缺氧影响细胞增殖和分化。全氟十四酸(PFTA)是一种很好的氧气载体。本研究通过EDC/NHS反应将PFTA负载到静电纺丝制备的聚己内酯和明胶复合纤维支架上以解决材料内部供氧不足的问题。首先对PFTA负载方法进行优化,确定EDC/NHS反应最佳溶剂为75%乙醇;其次考察和比较了材料的物化性能。PFTA修饰后的纤维形貌均匀,力学性能良好,并且随着PFTA含量的增加,材料亲水性略有下降。内皮细胞增殖实验结果显示,材料具有良好的细胞相容性。本研究为后期制备可募集氧气的人工血管奠定了基础。
Abstract：Insufficient oxygen delivery into tissue-engineered scaffolds affects tissue regeneration performance. Oxygen plays an important role in tissue regeneration. In engineered tissues, the cells in the central regions of scaffolds may be lack of oxygen inhibiting cell proliferation and differentiation. Perfluorotetradecanoic acid(PFTA) is a good oxygen carrier. Therefore, electrospun polycaprolactone(PCL) and gelatin hybrid fiber scaffolds loaded with PFTA were prepared to solve the insufficient oxygen supply problem in this study. On one hand, the PFTA loading method was optimized. The suitable solvent for the EDC/NHS reaction was 75% ethanol. On the other hand, the physical and chemical properties of prepared materials were investigated. The results showed that the material had uniform morphology, good mechanical properties and became relatively hydrophobic with the increasing concentration of PFTA. Endothelial cell proliferation assay results showed that the material had good cell compatibility The current results put way for the preparation of oxygen recruiting vascular grafts.
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基本信息:
DOI：10.16026/j.cnki.iea.2020030193
中图分类号:R318.08
引用信息:
[1]史杰,沙成花,吴静,等.全氟十四酸功能化修饰电纺聚己内酯/明胶多孔支架材料制备及评价[J].离子交换与吸附,2020,36(03):193-199.DOI:10.16026/j.cnki.iea.2020030193.
基金信息:
国家自然科学基金面上项目(81671842,31870966);; 国家重大专项(2017YFC 1103504);; 天津市自然科学基金重点项目(16JCZDJC37600)