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本文将大孔树脂分离纯化黄蜀葵花中总黄酮和多糖的工艺进行了优化，分离后能够同时得到含量较高的总黄酮和多糖两种附加值较高的产物。采用响应面实验方法确定大孔树脂最佳工艺为：从5种树脂中选用D101型大孔吸附树脂，黄蜀葵花粗提物以20%乙醇溶解，上样浓度为45mg/mL，上样流速2mL/min；洗脱时用5BV蒸馏水富集多糖；再用3BV 40%乙醇富集总黄酮。最终可同时得到纯度分别为67.33%和52.24%的总黄酮和多糖提取物，其两者含量分别提高了58.5%和18.0%。DPPH、ABTS和FRAP的抗氧化测定结果均表明抗氧化活性强度为：总黄酮提取物>多糖提取物>粗提物。该方法能够从黄蜀葵花提取物同时分离得到总黄酮和多糖两种产物，抗氧化活性测定结果也显示了富集后有更强的抗氧化活性，大大提高了黄蜀葵花中高附加产物的分离纯化效率，减少了分离过程中副产物的浪费，为黄蜀葵花高值化利用提供新的途径。
Abstract：Objective To obtain two high value-added products with higher content of total flavonoids and polysaccharides at the same time, the process of separation and purification of total flavonoids and polysaccharides from Abelmoschusmanihot flowers with macroporous resin was optimized. The optimal process of macroporous resin was determined by response surface experimental method: D101 macroporous adsorption resin was selected from five resins. The crude extract of Abelmoschusmanihot flowers was dissolved in 20% ethanol. The concentration of crude extract was 45 mg/mL and the loading flow rate was 2 mL/min. Polysaccharides were enriched with 5BV of distilled water during elution, and total flavonoids were enriched with 3BV of 40% ethanol. The total flavonoid and polysaccharide extracts with purities of 67.33%and 52.24% were obtained at the same time. The content of these two compounds increased by 58.5% and 18.0%, respectively. The results of DPPH, ABTS and FRAP showed that the antioxidant activity of total flavonoids extract was higher than polysaccharide extract and crude extract. This method could simultaneously isolated total flavonoids and polysaccharides from Abelmoschusmanihot flowers extract. The results of antioxidant activity also showed stronger antioxidant activity after enrichment, which greatly improved the separation and purification efficiency of high additional products in Abelmoschusmanihot flowers. This paper reduced the waste of by-products in the separation process, and provided a new way for the high-value utilization of Abelmoschusmanihot flowers.
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基本信息:
DOI：10.16026/j.cnki.iea.2023010036
中图分类号:R284.2
引用信息:
[1]谢青云,胥维昌,王远等.大孔树脂同时分离纯化黄蜀葵花总黄酮和多糖工艺优化[J].离子交换与吸附,2023,39(01):36-49.DOI:10.16026/j.cnki.iea.2023010036.
基金信息:
辽宁省揭榜挂帅项目“黑土地旱田地力提升关键技术示范与推广”; 辽宁省博士科研启动基金计划项目(2020BS275)