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为解决创伤修复过程中致病菌引起的感染问题,本研究制备了γ-聚谷氨酸/壳聚糖/纳米银复合水凝胶,对复合材料进行结构性能、物理性能、细胞毒性和抗菌性评价。透射电子显微镜结果表明,材料上合成粒径1~9nm的纳米银粒子;扫描电子显微镜观察到材料呈三维网状结构,傅里叶红外光谱表明,原位合成纳米银的化学反应不影响水凝胶的化学结构;热失重分析表明,材料具有良好的热稳定性且可以通过调整反应物剂量调控纳米银在水凝胶上的负载量;细胞毒性实验和抗感染实验证明复合水凝胶具有良好的细胞相容性和抗菌性。本研究为实现基于纳米银的抗菌性创伤修复材料的应用奠定实验基础。
Abstract：This study prepared the composite hydrogels composed of poly(γ-glutamic acide)(γ-PGA) and chitosan(CS).Silver nanoparticles(Ag NPs) were synthesized in the γ-PGA/CS hydrogels with the best swelling properties in situ.The structure and properties of the nanocomposite hydrogels were characterized by UV absorbance detection,Transmitting Electron Microscopy(TEM),Scanning Electron Microscopy(SEM),FT-IR and Thermo-gravimetric Analysis(TGA).The nanocomposite hydrogels exhibited a three-dimensional network structure and thermal stability.The in vitro antibacterial experiment revealed excellent antibacterial activity of the nanocomposite hydrogels against Escherichia coli and Staphylococcus aureus.The nanocomposite hydrogels would have great potential in antibacterial hydrogel and wound healing.
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基本信息:
DOI：10.16026/j.cnki.iea.2016040297
中图分类号:TQ427.26
引用信息:
[1]王建涛,潘晓晨,王淑芳.γ-聚谷氨酸/壳聚糖/纳米银复合水凝胶的制备和表征[J].离子交换与吸附,2016,32(04):297-305.DOI:10.16026/j.cnki.iea.2016040297.
基金信息:
国家重点基础研究发展计划(973计划)(2012CB725204);; 教育部“创新团队发展计划”(IRT13023);; 天津自然科学基金(16JCZDJC37600);; 药物化学生物学国家重点实验室开放基金(201603007);; 天津市大学生创新创业训练计划(201510055335)