离子交换与吸附

考察了HPD-826、HPD-417、ADS-17、HPD-722、HPD-450、AB-8、HPD-600、D-101,共8种大孔树脂对藏药白花龙胆花总黄酮的吸附和解吸性能,通过静态吸附量和解吸附率及静态吸附曲线的绘制,筛选出AB-8树脂的效果最佳;以AB-8树脂为目标,进行了动态吸附实验,考察了上柱液浓度、pH值、上柱液流速、乙醇浓度、解吸剂流速、解吸体积等对AB-8树脂吸附和解吸效果的影响,确定出AB-8树脂动态吸附白花龙胆花总黄酮的最佳条件:上柱液浓度为6.5mg/mL,pH为3.79,上柱流速4BV/h;最佳洗脱条件:用50%乙醇进行洗脱,解吸流速为3BV/h,解吸体积4BV。在此条件下,白花龙胆花总黄酮纯度由原来的22.10%,变为65.75%,产品精制倍数为65.75%/22.10%=2.97,表明AB-8树脂可用于白花龙胆花总黄酮的分离纯化。

The characteristics of macroporous adsorption resins HPD-826,HPD-417,ADS-17,HPD-722,HPD-450,AB-8,HPD-600 and D-101 on adsorption and desorption of flavonoids in the flower of Gentiana tizuensis Franch were investigated.Based on static adsorption and desorption assay,the macroporous resin AB-8 was chosen to be the best.Then the favourite resin AB-8 was chosen to carry out dynamic adsorption experiments.The evaluation indexes included extract concentration,pH,adsorption velocity,eluting reagent,desorption rate and desorption dose.The results indicated that the optimum condition:the extract concentration was 6.5mg/mL,the pH value was 3.79,adsorption velocity was 4BV/h,the eluting reagent was 50% ethanol,the desorption rate was 3BV/h,the dose of the reagent was 4 times as much as the column volume.Under these conditions,the purification of flavonoids was 66.75%,which was 2.97 times higher than before.The results showed that AB-8 resin could be used in separation and purification for total flavone in the flower of Gentiana tizuensis Franch.