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比较了不同pH值下预处理萝卜硫素溶液对树脂吸附量的影响,测定了SP850树脂对萝卜硫素的吸附动力学曲线和不同温度下的等温吸附线。实验结果表明,在pH=3下进行预处理,能有效除去蛋白质等杂质,树脂吸附量最大;SP850树脂对萝卜硫素的吸附速率快,120min就达到吸附平衡,吸附量为77.28mg/g树脂。吸附动力学曲线符合准二级吸附动力学模型,吸附过程以液膜扩散为控制步骤;SP850树脂对萝卜硫素的等温吸附线可用Freundlich方程拟合,低温有利于吸附。以上研究结果可为萝卜硫素的分离提供理论依据。
Abstract：The adsorption performance of pretreatment of sulforaphane solution at different pH value were compared. The adsorption kinetics and adsorption isotherms of sulforaphane adsorbed onto SP850 resins were studied. The experiment results showed that when the sulforaphane solution was pretreated at pH 3, protein and other impurities could be effectivelyremoved and the adsorption capacity was higher. The adsorption rate of sulforaphane adsorbed onto SP850 resins was quick. Within 120min, the adsorption equilibrium could be reached and equilibrium adsorption capacity was 77.28mg/g resin. The data of adsorption kinetics of sulforaphane onto SP850 resin could be correlated with Pseudo-second-order model so that the adsorption process was mainly controlled by liquid film diffusion. The adsorption of sulforaphane onto SP850 resins at different temperature could be described by Freundlich isotherm model, and low temperature tended to favor to adsorption process These research results provided a theoretical reference for purification of sulforaphane.
[1]王见冬,袁其朋,钱忠明.萝卜硫素研究进展.食品与发酵工业[J].2002,29(2):76-80.
[2]张婵娟,郭晓玲,孟青,等.不同品种西兰花种子中莱菔硫烷的HPLC分析.广东药学院学报[J].2007,23(5):506-508.
[3]Joell J Gills,Elizabeth H Jeffery,Nathan V Matusheski,et al.Sulforaphane prevents mouse skin tumorigenesis during the stage of promotion.Cancer Letters[J],2006,236(1):72-79.
[4]Thejass P,Kuttan G.Antimetastatic activity of sulforaphane.Life Sciences[J].2006,78(26):3043-3050.
[5]Thejass P,Kuttan G.Immunomodulatory activity of sulforaphane,a naturally occurring isothiocyanate from broccoli(Brassica oleracea).Phytomedicine[J].2007,14(7/8):538-545.
[6]刘锡建,肖稳发,曹俭,等.SP850树脂分离萝卜硫素.食品与发酵工业[J].2011,37(7):197-200.
[7]苏光耀,沈莲清,王向阳,等.西兰花籽中硫代葡萄糖苷酶解条件的研究.中国粮油学报[J].2008,23(2):178-182.
[8]Ho Y S,McKay G.Pseudo-second order model for sorption processes.Process Biochemistry[J].1999,34(5):451-465.
[9]杨小彦,李稳宏,唐璇,等.胡芦巴总皂苷在HPD 400A大孔树脂上的吸附性能研究.离子交换与吸附[J].2011,27(1):53-60.
[10]曾小伟,陈丽娟,范云鸽,等.一种后交联大孔吸附树脂的合成及其对苯酚和维生素B12吸附性能研究.高分子学报[J].2009,(9):908-916.
[11]杨国栋.花生壳吸附水中Cr6+的研究.离子交换与吸附[J].2012,28(4):369-374.
[12]康家胜,方菊,魏凤玉,等.大孔树脂对竹叶总黄酮的吸附平衡和动力学.化工进展[J].2011,30(10):2158-2162,2177.
[13]杨鹏波,张晓燕,丛威.大孔吸附树脂吸附乳酸及乳酸与谷氨酸的分离.过程工程学报[J].2007,7(4):767-771.
[14]刘锡建,肖稳发,谢燕婷,等.D-101树脂对胭脂虫红色素吸附特性的研究.离子交换与吸附[J].2012,28(1):78-85.
[15]马宏瑞,韩晓晶,王家宏.有机改性凹凸棒土对腐殖酸的吸附性能研究.离子交换与吸附[J].2012,28(1):46-53.
基本信息:
DOI：10.16026/j.cnki.iea.2013.06.004
中图分类号:O647.3
引用信息:
[1]刘锡建,王明强,徐一麟,等.SP850树脂对萝卜硫素吸附特性的研究[J].离子交换与吸附,2013,29(06):561-568.DOI:10.16026/j.cnki.iea.2013.06.004.
基金信息:
上海工程技术大学科技发展基金(2011Q12);; 上海工程技术大学大学生创新项目(cx1104034、cx1204020)
2013-12-20
2013-12-20