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采用大孔吸附树脂分离纯化拳参黄酮,筛选其与VC协同抗氧化最佳活性部位。以静态吸附率和解吸率为指标,从中筛选出富集拳参黄酮的最佳树脂,研究其吸附热力学特性,并以黄酮液p H值、浓度等性能确定其动态吸附、洗脱条件。结果表明,NKA-II大孔吸附树脂对拳参黄酮的吸附可用Freundlich方程进行描述,此过程为优惠吸附过程。NKA-II大孔吸附树脂纯化拳参黄酮的最佳条件为:以p H值为2,0.4296mg/m L黄酮液上样,使用40%、60%、80%、95%乙醇溶液洗脱。通过·DPPH清除实验和IC50mix值、相互作用指数等对各复配物抗氧化性进行评价,其中95%乙醇洗脱部位与VC以1:2复配比组成的复配组,协同抗氧化效果最佳。实验结果说明,拳参黄酮是一种很好的天然抗氧化剂。
Abstract：Favonoids of Polygonum bistora L. were purified by macroporous adsorption resins, then the active fraction of flavonoids with synergistic antioxidant combined with VC was screened. Based on the experimental results of static adsorption efficiency and desorption efficiency, the suitable resin was chosed, and the adsorption thermodynamics of flavonoids in solution was studied. The effect of p H value and concentration of flavonoids on adsorption conditions were determined. The results showed that the isothermal adsorption of flavonoids on NKA-II macroporous resin was described well by Freundlich equation, and the process was a preferential adsorption. The optimum purification conditions of flavonoids were as follows: the sample was 0.4296mg/m L of flavonoids at p H 2, the concentration of ethanol used as eluent was 40%, 60%, 80%, 95%, respectively. Meanwhile, the integration of flavonoids extracted by 95% ethanol and VC(1:2) exhibited a remarkable synergistic antioxidation in terms of scavenging ·DPPH, IC50 mix and the interaction index. All results showed that the flavonoids of Polygonum bistora L. was an excellent natural antioxidation product.
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基本信息:
DOI：10.16026/j.cnki.iea.2015030230
中图分类号:R284.2
引用信息:
[1]李帅,刘军军,马兴元,等.拳参黄酮与VC协同抗氧化活性部位的筛选[J].离子交换与吸附,2015,31(03):230-241.DOI:10.16026/j.cnki.iea.2015030230.
基金信息:
国家自然科学基金项目(No.C020407);; 河西学院大学生科技创新项目(No.103)