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通过血液净化来治疗耐药或家族性高脂血症越来越受关注,而目前尚未有国产低密度脂蛋白吸附柱上市。因此,开展高脂血症吸附剂的研究具有重要的理论和现实意义。本研究以大孔聚乙烯醇微球为载体,经超声作用成功制备聚乙烯醇-羧基化多壁碳纳米管复合吸附剂,拉曼光谱证实了含羧基化多壁碳纳米管复合微球的成功制备。体外静态/动态条件下对吸附性能进行了系统性评价。结果表明,聚乙烯醇-羧基化多壁碳纳米管复合型吸附剂对低密度脂蛋白、总胆固醇、甘油三酯和高密度脂蛋白的饱和吸附量分别为(1.64±0.01)mg/mL,(2.13±0.02)mg/mL,(0.69±0.01)mg/mL和(0.23±0.02)mg/mL;吸附动力学曲线显示吸附平衡时间为3h;血清中离子强度的增大会降低复合型吸附剂对血脂中低密度脂蛋白和总胆固醇的吸附性能,表明静电相互作用在聚乙烯醇-羧基化多壁碳纳米管吸附剂与低密度脂蛋白的吸附中起主要作用。体外循环条件下可有效清除有害血脂成分。灭菌后聚乙烯醇-羧基化多壁碳纳米管吸附剂仍保持较高的吸附性能;正常条件下储存12w对吸附性能基本没影响;复合微球溶血率低,对全血成分影响不显著且细胞毒性为0级。本研究为纳米复合型吸附剂的制备和临床应用提供了实验基础与参考。
Abstract：The treatment of drug resistance or familial hyperlipidemia by blood purification has attracted increasing attention. At present, there is no domestic low density lipoprotein adsorption column. Therefore, the study of hyperlipidemia adsorbent has important theoretical and practical value. In this study, the composite adsorbent of polyvinyl alcohol-carboxylic multiwalled carbon nanotubes was successfully prepared by ultrasonic with macroporous polyvinyl alcohol microspheres as the carrier, which was confirmed by Raman spectroscopy. The adsorption properties were systematically evaluated under static/dynamic conditions in vitro. The results showed that the saturated adsorption capacity of the composite adsorbent for low density lipoprotein, total cholesterol, triglyceride and high-density lipoprotein was(1.64±0.01)mg/mL,(2.13±0.02)mg/mL,(0.69±0.01)mg/mL and(0.23±0.02)mg/mL, respectively. The adsorption kinetic curve showed that the adsorption equilibrium time was 3 h. The increase of ionic strength in serum would reduce the adsorption performance of compound adsorbent on low density lipoprotein and total cholesterol in blood lipids, indicating that electrostatic interaction played a major role in the adsorption of low density lipoprotein. The harmful lipids can still be removed effectively in the extracorporeal circulation. Storage for 12 weeks under normal conditions has little effect on adsorption performance. The hemolysis rate of the composite microspheres was low, and the effect of the polyvinyl alcohol-carboxylic multiwalled carbon nanotubes composite adsorbent on the whole blood components was not significant, the cytotoxicity was grade 0. This study provides an experimental basis for the clinical application of nano-composite adsorbent.
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基本信息:
DOI：10.16026/j.cnki.iea.2021040323
中图分类号:R318.08;TQ424
引用信息:
[1]宗文辉,王力淳,李毅敏,等.新型聚乙烯醇-羧基化多壁碳纳米管复合型吸附剂对低密度脂蛋白的吸附性能研究[J].离子交换与吸附,2021,37(04):323-334.DOI:10.16026/j.cnki.iea.2021040323.
基金信息:
国家自然科学基金面上项目(No.81771986)
2021-08-20
2021-08-20