北京石油化工学院新材料与化工学院,燃料清洁化及高效催化减排技术北京市重点实验室;
肝素亲和层析是利用生物大分子与层析介质表面的肝素特异性结合而进行选择性分离的一种技术,使用条件温和,纯化过程中能较好保持目标蛋白的生物活性。本文在阐述肝素与层析介质化学反应机理的基础上,系统论述了溴化氰活化及偶联法、环氧活化及偶联法、席夫碱法和碳二亚胺缩合法四种肝素亲和层析介质制备方法,深入分析了各制备方法的优缺点,总结了肝素亲和层析在血液制品、病毒纯化方面的应用。最后,进一步分析了肝素亲和层析在蛋白分离纯化领域应用存在的问题,并展望了其未来发展趋势,以期为肝素亲和层析介质的制备方法提供新思路。
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基本信息:
DOI:10.16026/j.cnki.iea.2023020172
中图分类号:O629.73
引用信息:
[1]古嘉如,穆士凯,武玉哲等.肝素亲和层析介质制备方法及应用研究进展[J].离子交换与吸附,2023,39(02):172-188.DOI:10.16026/j.cnki.iea.2023020172.
基金信息:
北京市委组织部青年拔尖人才项目(ZZB2019001)