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茶皂素具备多种生物活性，已被应用于各个领域，但现阶段提取出的茶皂素纯度较低，不利于进一步开发利用，故需要对其进行分离纯化。从18种大孔吸附树脂中筛选出纯化茶皂素性能最佳的树脂，分析其吸附热力学和吸附动力学特征，并考察其纯化前后体外抗氧化活性差异。结果表明，在18种大孔吸附树脂中，X-5型树脂对茶皂素的纯化效果是最佳的，其得率为(84.1±0.07)%;Langmuir等温吸附模型(R2>0.99)较Freundlich模型能够更好地拟合茶皂素在X-5型树脂上的吸附等温线；X-5型树脂对茶皂素的吸附过程属于自发进行、熵减、放热的物理吸附，更适于采用准二级动力学模型(R2>0.99)进行拟合；经X-5型树脂纯化后，茶皂素的纯度(24.18±0.3)%提升至(80.62±0.42)%，清除ABTS自由基、DPPH自由基的能力和总还原力分别是纯化前的1.63、1.61和1.57倍，说明纯化效果显著。
Abstract：Tea saponins have various biological activities and can be applied in various fields. However, at present, the extracted tea saponins generally have low purity, therefore they are not applicable for further development and utilization, so the isolation and purification are required. This study selected the resin with the best performance in purifying tea saponins from 18 types of macroporous adsorption resins, analyzed its adsorption isotherms, adsorption thermodynamics and adsorption kinetics characteristics, and the difference of antioxidant activity between before and after purification was investigated. The results showed that the kind of X-5 macroporous adsorption resin had the best purification effect among the 18 selected macroporous adsorption resins, with the yield of(84.1±0.07) %; The adsorption thermodynamics of tea saponins can be better fitted by the Langmuir model(R2>0.99), compared with Freundlich model; The adsorption process of tea saponins by the kind of X-5 macroporous adsorption resin belonged to spontaneous, entropy reducing, and exothermic physical adsorption. Furthermore, it is more suitable to use the quasi second-order kinetic equation(R2>0.99) for fitting;After purification by X-5 macroporous adsorption resin, the purity of tea saponins increased from(24.18±0.3) %to(80.62±0.42) %; The ability of purified tea saponins to scavenge ABTS free radicals, DPPH free radicals, and total reducing power increased to 1.63 times, 1.61 times, and 1.57 times, respectively, before purification, which indicated that the purification effect was significant.
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基本信息:
DOI：10.16026/j.cnki.iea.2024030255
中图分类号:TQ423.2;O647.3
引用信息:
[1]尹任焰,杨佳瑶,王艺潼等.大孔吸附树脂对茶皂素的吸附纯化特性及抗氧化活性研究[J].离子交换与吸附,2024,40(03):255-263.DOI:10.16026/j.cnki.iea.2024030255.
基金信息:
浙江省“领雁”攻关研发计划项目(基金号2022C02023); 中央本级重大增减支项目(基金号2060302); 浙江科技学院科研业务专项(基金号2023JLZD007,2023QN024)