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光动力治疗(PDT)因其具有非侵入性、时空可控性、广谱抗菌、不易诱导耐药性等优点，在耐药菌杀伤领域受到广泛关注。与传统光敏剂不同，具有聚集诱导发光(AIE)性质的光敏剂在聚集态时仍具备产生大量活性氧物种的能力。然而，经典的给体-受体(D-A)型阳离子AIE光敏剂具有较大的暗毒性。针对该问题，本文设计合成了一种具有D-A结构的非电荷型AIE光敏剂(MTTCN)，基于混合电性聚合物体系实现了MTTCN的有效纳米封装，制备得到了表面带正电荷的纳米颗粒(MTTCN@PVA/PAH)。实验结果表明，MTTCN@PVA/PAH具有良好的生物相容性和对负电性细菌的结合能力，在白光辐照下对耐甲氧西林金黄色葡萄球菌表现出高效的光动力杀伤效果，在耐药菌感染治疗中具有潜在的应用价值。
Abstract：Photodynamic therapy(PDT) has attracted much attention in the treatment of drugresistant bacteria due to its non-invasiveness, spatiotemporal controllability, broad-spectrum killing effects, and less probability to induce drug resistance. Different from traditional photosensitizers, aggregation-induced emission(AIE) type photosensitizers can sensitize the generation of considerable reactive oxygen species(ROS) even in the aggregate state. However,most donor-acceptor(D-A) type cationic AIE photosensitizers show evident toxicity to biological systems. To overcome this issue, we design and synthesize a nonionic D-A type AIE photosensitizer(denoted as MTTCN), which is followed by formulation into positively charged nanoparticles with the aid of two polymers with different charges(denoted as MTTCN@PVA/PAH). Experimental results show that MTTCN@PVA/PAH shows good biocompatibility and effective binding with negatively charged bacteria. These features enable MTTCN@PVA/PAH to exhibit outstanding killing effects against methicillin-resistant Staphylococcus aureus under white light irradiation, holding great potential in the treatment of drug-resistant bacterial infections.
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基本信息:
DOI：10.16026/j.cnki.iea.2023040368
中图分类号:R313;TB383.1
引用信息:
[1]武凯宇,孙振程,符豪,等.非电荷型AIE光敏剂的纳米封装及光动力抗菌研究[J].离子交换与吸附,2023,39(04):368-380.DOI:10.16026/j.cnki.iea.2023040368.
基金信息:
国家自然科学基金(No.92163126,52003123)
2023-08-20
2023-08-20