| 809 | 7 | 100 |
| 下载次数 | 被引频次 | 阅读次数 |
以壳聚糖(CS)和β-环糊精(β-CD)为原料,制备了壳聚糖接枝β-环糊精聚合物(CS-g-CD),然后以三聚磷酸钠(TPP)为交联剂,通过静电自组装的方法得到了聚合物空心微球,并以5-氟尿嘧啶(5-FU)为模型药物,研究了聚合物空心微球对药物的负载性能。利用红外光谱(FT-IR)、元素分析、X-射线衍射(XRD)表征了聚合物的结构和组成,激光粒度仪和透射电子显微镜(TEM)表征了聚合物微球的粒径及形貌。通过紫外可见分光光度计研究了微球对药物的载药量和包封率。结果表明,得到的微球呈现较为规则的空心结构,粒径在300~350nm左右,分散性较好,对药物载药量和包封率较高。
Abstract：The CS-g-CD polymer was readily synthesized from chitosan and mono-6-deoxy-6-(p-toluenesulfonyl)-β-cyclodextrin. Then polymer hollow microspheres were prepared by electrostatic self-assembly using sodium tripolyphosphate(TPP) as cross-linking agent. At the same time, 5-fluorouracil(5-FU) was selected as a model drug to investigate the properties of the assemblies. The structure, particle size, morphology, encapsulation efficiency and loading efficiency were characterized and investigated by fourier transform infrared spectroscopy, elemental analysis, X-ray diffraction, laser diffraction particle size analysis, transmission electron microscopy and UV-visible spectroscopic techniques. The results revealed that the microspheres were uniform hollow spherical in shape with particle size between 300~350nm and good size distribution. The CS-g-CD microspheres had high loading efficiency and high entrapment efficiency for 5-FU.
[1]Mu B,Peng L,Li X,et al.Fabrication of flocculation-resistant p H/ionic strength/temperature multiresponsive hollow microspheres and their controlled release.Molecular Pharmaceutics[J].2011,9(1):91-101.
[2]Ke C J,Su T Y,Chen H L,et al.Smart multifunctional hollow microspheres for the quick release of drugs in intracellular lysosomal compartments.Angewandte Chemie[J].2011,50(35):8086-8089.
[3]Such G K,Johnston A P R,Caruso F.Engineered hydrogen-bonded polymer multilayers:from assembly to biomedical applications.Chemical Society Reviews[J].2011,40(1):19-29.
[4]张停,李桂英,李睿鑫,等.温度敏感性壳聚糖基聚电解质载药纳米粒子的制备及表征.离子交换与吸附[J].2012,28(6):524-531.
[5]金政,刘美华,刘振梅,等.壳聚糖缓释体系研究进展.化学与黏合[J].2015,37(1):65-71.
[6]Park J H,Yong W C,Son Y J,et al.Preparation and characterization of self-assembled nanoparticles based on glycol chitosan bearing adriamycin.Colloid and Polymer Science[J].2006,284(7):763-770.
[7]Berrada M,Serreqi A,Dabbarh F,et al.A novel non-toxic camptothecin formulation for cancer chemotherapy.Biomaterials[J].2005,26(14):2115-2120.
[8]Brewster M E,Loftsson T.Cyclodextrins as pharmaceutical solubilizers.Advanced Drug Delivery Reviews[J].2007,59(7):645-666.
[9]Dass C,Jessup W.Apolipoprotein A-I,Cyclodextrins and liposomes as potential drugs for the reversal of atherosclerosis.A review.Journal of Pharmacy and Pharmacology[J].2000,52(7):731-761.
[10]荆鹏飞,刘慧君,胡趁,等.β-环糊精衍生物的制备方法及其应用研究进展.精细化工中间体[J].2014,44(6):1-4.
[11]Khan A R,Forgo P,Stine K J,et al.Methods for selective modifications of cyclodextrins.Chemical Reviews[J].1998,98(5):1977-1996.
[12]Anirudhan T S,Divya P L,Nima J.Synthesis and characterization of novel drug delivery system using modified chitosan based hydrogel grafted with cyclodextrin.Chemical Engineering Journal[J].2016,284(15):1259-1269.
[13]Yuan Z,Ye Y,Feng G,et al.Chitosan-graft-β-cyclodextrin nanoparticles as a carrier for controlled drug release.International Journal of Pharmaceutics[J].2013,446(1/2):191-198.
[14]Chen Y Z,Huang Y K,Chen Y,et al.Novel nanoparticles composed of chitosan andβ-cyclodextrin derivatives as potential insoluble drug carrier.Chinese Chemical Letters[J].2015,26(7):909-913.
[15]Samuels R J.Solid state characterization of the structure of chitosan films.Polymer Physics[J].1981,19(7):1081-1105.
[16]Xing J,Deng L,Dong A.Chitosan/alginate nanoparticles stabilized by poloxamer for the controlled release of 5-fluorouracil.Applied Polymer[J].2010,117(4):2354-2359.
[17]Yu N,Li G,Gao Y,et al.Thermo-sensitive complex micelles from sodium alginate-graft-poly(N-isopropylacrylamide)for drug release.International Journal of Biological Macromolecules[J].2016,86:296-301.
[18]Zheng Y,Yang W,Wang C,et al.Nanoparticles based on the complex of chitosan and polyaspartic acid sodium salt:preparation,characterization and the use for 5-fluorouracil delivery.European Journal of Pharmaceutics and Biopharmaceutics[J].2007,67(3):621-631.
基本信息:
DOI：10.16026/j.cnki.iea.2016050414
中图分类号:TQ460.4;O636.1
引用信息:
[1]高玉荣,于娜娜,李桂英.壳聚糖接枝β-环糊精聚合物空心微球的制备及其载药性能[J].离子交换与吸附,2016,32(05):414-422.DOI:10.16026/j.cnki.iea.2016050414.
基金信息:
国家自然科学基金项目(21204035)资助