离子交换与吸附

以新型生物聚酯P3HB4HB静电纺丝纤维支架材料为基质,通过层-层自组装技术向基质中引入γ-聚谷氨酸和聚赖氨酸,并负载含硒催化剂硒代胱胺,构建具有催化NO原位生成功能的人工血管材料,通过体外催化释放NO实验、细胞毒性检测、平滑肌细胞铺展实验考察人工血管材料的生物功能。实验结果表明,该材料具有良好的纤维多孔结构,适于作血管支架材料;通过控制有机硒的负载量可调控NO催化生成速率;而且该材料具有良好的生物相容性,能够有效抑制平滑肌细胞的黏附与铺展。

Small-diameter vascular grafts are increasingly needed in the clinic. In this paper, a novel vascular graft was constructed in situ catalytic nitric oxide generation. Poly(γ-glutamic acid) and poly(α-lysine) were alternatively deposited onto the surface of electrospun P3HB4 HB matrix via electrostatic layer-by-layer self-assembly, and then selenocystamine was loaded as a catalyst. The biological functions of the vascular grafts were tested via determination of nitric oxide generation, cellular proliferation experiment and spreading of smooth muscle cell experiment. As a result, the fibrous structure with pores of electrospun P3HB4 HB was suitable as scaffold of vascular grafts. The results of determination of in vitro catalytic nitric oxide generation demonstrated that this catalyst-loaded material could significantly accelerate the release of nitric oxide from S-nitrosoglutathione. The material possessed good cellular compatibility. Besides, the catalyst-loaded material exhibited the capability of inhibiting smooth muscle cell spreading.